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v28 Study Germplasm

v28 Study Germplasm

About

The list of germplasm to be used in the study, and role (or type) of germplasm entries in the context of the study (Test, Check/Control, etc), and entry details are defined in the Germplasm and Checks tab.

Add Germplasm Descriptors

Notice the default list of entry details that will be included in the study.  Entry details are list-specific and have been added via v28 Germplasm Lists | Add and Remove Entry Details. Once added, the information can be selected to appear in this section too.

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Additional entry details can be selected by clicking the + button.

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Select Entry Details from the drop-down list

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New Entry detail variables are defined in Ontology Manager (see v28 Manage Ontologies | Add & Edit Variable )

Choose Germplasm List

Click Browse to select the germplasm list for use in the study.

Actions

Click on Actions to reveal options for:

  • replacing germplasm entries in the study

  • adding new germplasm entries in the study

  • importing germplasm entry details relevant to the study

  • setting germplasm entry types specific to the study

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Replace Germplasm

  • Tick the germplasm for replacement.

  • Go to Actions, then Replace Germplasm.

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  • Apply filters to find germplasm of interest to use as the replacement. Click Select.

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If the experimental design has already been generated, you will need to confirm the replacement.

Add New Entries

  • Select Add New Entries. Use the germplasm filters to find germplasm of interest to add to the study. Note: If the experimental design has already been generated, the new entry will not appear until the previous design is deleted and the design regenerated.

Import Entry Details

This action allows to add more entry details, if it haven’t been added yet in Germplasm Lists, to be displayed in the studybook.

Download the template. Select additional variable from Entry Details sheet, ex. Entry_code2.

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Fill it with values in Observation sheet.

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Click Actions > Import Entry Details and browse through the template. The newly added entry detail is shown as another column.

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Set Entry Type

By default, all germplasm entries in the study are assigned with type T (“Test entry”), however a set of germplasm may be specified as controls or checks with type C (“Check entry”).

The table below contains the default entry types.

Any additional custom, optional entry types can be defined by a user with Manage Ontologies permission.

Abbreviation of ENTRY_TYPE

Description

Notes

Abbreviation of ENTRY_TYPE

Description

Notes

T

Test entry

 

C

Check entry

 

X

Non Replicated

For identifying which entries will not be replicated in a P-Rep design

D

Disease check

 

S

Stress check

 

R1

Replicated entry in family 1

For identifying the 1st family/group of sister lines; treated as a “Test entry“ for P-Rep design and as “Check entry” for other designs

R2

Replicated entry in family 2

For identifying the 2nd family/group of sister lines; treated as a “Test entry“ for P-Rep design and as “Check entry” for other designs

R3

Replicated entry in family 3

For identifying the 3rd family/group of sister lines; treated as a “Test entry“ for P-Rep design and as “Check entry” for other designs

If the experimental design has been generated, the Set Entry Type option will not be available from the Actions menu. You must delete the experimental design to change the entry type.

Single entry

Click on the individual cell under ENTRY_TYPE column to change the type of a single entry. Choose the appropriate type from the dropdown list.

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In batch

Tick multiple entries that need an entry type change in one go. Click on Actions then select Set Entry Type from the drop-down list.

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Select the appropriate Entry Type. Click Accept.

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About checks

Experimental design will determine the placement of checks.

Randomized Check Distribution

If you choose a randomized experimental design, checks will be distributed randomly (see Experimental Design).

The two check entries will be randomized within each replication

 

Randomized Complete Block Design with 25 test entries, 2 checks, 1 environment, and 3 replications.

 

Uniform Check Distribution (Non-Random)

Non-randomized designs, based on entry list order, allow for the specification of uniform check interval spacing (see Experimental Design).

Two checks inserted uniformly at each position between a set of 10 test entries


Entry List Order design with 25 test entries, 2 checks, 1 environment, and 0 replications.

Columns

Click the Columns button to reveal various germplasm descriptors than can be included in the studybook (or fieldbook).

Additional information such as Cross, GroupID, Female & Male Parents, Attributes and Passport details can appear in the studybook whenever such information are available for the germplasm.

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Select the necessary descriptors by ticking the corresponding boxes.

 

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Click Apply.

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Observation sheet in Excel studybook

 

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