About

Advancements of selected germplasm generally use maintenance or derivative breeding methods - resulting in offspring less than or equal to the parent(s) in genetic diversity. Advancement can be made by plot or within plot selections. A breeder will chose to advance germplasm for a variety of reasons. Examples include:

• Advance F1 generation to create GIDs for F2 offspring

• Advance plants of interest to create GIDs for the offspring or clones

• Advance inbred lines to create GIDs for bulked seeds

• Advance a bag of seed to create GIDs for individual seeds/seedlings  

Advance by Plot

The following example every plot will be advanced, such as for a seed bulk.

• Open a study observations tab. Select Advance Study from the Actions menu > Advance study options.

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• Select which of the study instances (locations in this case) to advance. Continue.

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• Choose a breeding method and Finish. Derivative and maintenance breeding methods are filtered by default since these are the most common for advancements. If a bulking method like Seed increase bulk is chosen, all plots are selected by default. There’s an option to select entries from all or specific reps only.

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• Review the advanced lines and select Finish.

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Notice that the pending advanced lines have been automatically named by adding the season (Year) to the initial designation/name of the line. In this example, the initial line name was IBP2505, and after advancing by seed increase bulk method, the new name is IBP2505-2025. This is the default naming convention associated with the breeding method, Seed increase bulk. See your system administrator if you would like to change the default naming conventions.

Specify the folder where the list will be saved. Name the list and add optional information. Save.

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Advanced study using the Seed increase (bulking method). The advanced lines are populated in the Crosses and Selections tab within the study.

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Within Plot Selections

Within plots, selections are made based on phenotypic or genotypic criteria. To make within-plot selections, the study needs a Selection Variate with selections recorded.

No Plant ID

Select and advance plants within a plot.

• From the Actions menu > Advance study options, choose Advance Study. In this example, number of plants selected (NPSEL) is a selection variate.

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The lines will be advanced based on the selection variate (NPSEL-Number of plants selected). A single plant from each plot with disease resistance of scores between 0-3 has been selected for advancement.

• Select which of the study instances (location in this case) to advance. Continue.

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• Choose a breeding method to be used in advancing. Derivative and maintenance breeding methods are filtered by default since these are the most common for advancements. Deselect 'Same number of lines is selected for each plot'. Choose the selection variate that defines the number of lines advanced from each plot. Finish.

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• Review the advanced lines and select Finish.

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Notice that the pending advanced lines have been automatically named. For example, the designation, IBP2506-B1-1, is a concatenation of the line name(IBP2506-B1) and the plant sequence number, '1'. This is the default naming convention associated with the breeding method, single plant selection. See your system administrator if you would like to change the default naming conventions.

• Specify the folder where the list will be saved. Name the list and add optional information. Save.

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With Plant ID

If you need a unique identifier (UID) for individual plant observations, create a plant sub-observation data set (see more in Observations & Sub-Observations). The plant sub-observation UIDs are suitable for barcoding individual plants within a plot. Advance from the plant sub-observation dataset.

Crosses and Selections Tab

The advancing populates the Crosses and Selections tab.  Additional crosses and selections from this nursery can be collated in this table.  You are now able to harvest Crosses and Selections by first creating lots. See more in Manage Inventory.

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