This tutorial series describes how to design and manage a Breeding Management System (BMS) study planted with five doubled haploid (DH) maize lines. The scenario follows five promising DH maize lines newly introduced to a maize breeding program. The lines will be planted in a study of five non-randomized plots. The DH lines planted in the study will provide the basis for three activities.

本系列教程介绍了在育种管理系统 (BMS)中，如何对五种双单倍体 (DH) 玉米品系进行设计和管理的研究。本研究是将五种有潜力的 DH 玉米品系引入玉米育种项目中。这些品系将在五块非随机地块中种植。本研究中种植的 DH 品系将为三项活动提供研究基础。

1. Genotyping of the DH lines for assurance that the lines represent five unique genotypes and contain markers of interest.
   (对 DH 系进行基因分型，以确保该品系代表五种独立的基因型并包含感兴趣的标记.)
   
   

2. Design a diallel crossing scheme for DH lines (including reciprocals and excluding selfs) to create hybrid seed. The hybrid seed is destined for a future study - a randomized performance trial.
   (设计 DH 系（包括相互杂交和排除自交）的双列杂交方案以生成杂交种。杂交种将用于进一步的研究 – 随机区组试验。)
   
   

3. Increase the DH seed by advancement.
   (通过高级试验进行DH种子扩繁)

Tutorial Steps (教学步骤)

1. Review the DH lines to be used in the study.
   (确认研究中使用的 DH 系)

2. Design study: 5 plots, single location, non-randomized.
   (设计研究：5 个地块，单一地点，非随机化。)

3. Design diallel crosses and generate hybrids in the database. Crosses are generative breeding methods expected to increase genetic diversity in offspring relative to the parents. 
   (设计双列杂交并在数据库中生成杂交种。杂交是一种育种方法，有望增加后代相对于亲本的遗传多样性。)

4. Increase DH seed by advancing selfed ears to the next generation.
   (通过将自交穗自交一代，增加 DH 种子量)