Pedigree nurseries are managed through a series of nurseries facilitated through the Study Manager.

Objectives

At the end of this tutorial, the user should be able to:

1. Create a nursery study for population development

2. Advance a pedigree nursery by derivative methods

3. Withdraw seed from inventory and prepare planting labels

Create an F1 Nursery

Open the Manage Studies application from the main menu and click on Start a new study. Name the nursery <your initials>21F1, CGM21F1 for me, fill in the basic details of the description and objective, and select the study type Nursery.

 

 
You can add variables to the new study directly from the Ontology pick lists as we did for the crossing block in a previous Tutorial, or you can pick up all the variables from any previously used study. To use this option check the Use a previously created study as a template check box. Then choose the previous study that is similar to your current study:

 

Save the study in your 2021 Nursery folder.

On the Germplasm and Checks tab, the extra variables for Cross recovered from the template, so you just need to Browse for the list of planting material. The F1 list you made in the crossing Tutorial. U21CGMF1 in my case.

In the Environment tab, Mbe has been inherited from the template, and this location is still correct, but a new seeding date should be entered if known.

On the Experimental Design tab select Entry list order and Generate the design.

Advance the F1 Nursery by bulk selection of all families

Once the nursery is planted and harvested we can advance the families to F2 seed by bulking seed from each F1 cross family. Select Actions>Advance study options>Advance study:

Choose the location to advance (we only have one) - click Continue. 

 

 

In the Propagate Descriptors, click Continue

 

 

then select the advanced method for all plots – Random Bulk - DRP. We will advance all plots so leave the All plots are selected checkbox ticked.

 

Review the list of advanced lines by clicking on the Preview button, and if it looks correct
Click Advance.

Save the list with the name <your initials>21F2 in your 2021 lists folder.

 

You can follow the steps in the tutorial Adding seed inventory for a harvest list to add inventory for the F2 seeds if you would like.

Create an F2 nursery and load some data from a field book

 

Follow the steps to create a new nursery for the F2 seeds. Use the F1 nursery as a template for planting at Mbe in Entry list order. (You can review the steps in the first section above).

 

In this nursery, we will need to add a variable to record the number of plants selected from each F2 population.
If the flowering date is not a column in the Observation sheet, click Add opposite Traits search for flowering, and add FlwDate_50Flw_Date as a trait.

Click the Add button opposite SELECTIONS on the Observation tab and look for the variable NPSEL to add to the fieldbook.

Now we can export the fieldbook for data collection. Select Actions>Data collection options>Export study book:

Choose Observations and then choose Excel Format.
You will download an Excel fieldbook with a description sheet:

And an Observation Sheet. Fill in the data for the Flowering Date (dates are entered as numbers in format YYYMMDD) and the number of plants selected from each plot:

Back in BMS select Actions>Data collection options>Import observations.

Press Continue for the observations sheet, then browse to the fieldbook file you just saved.

When you have chosen the file click Import.

The data will be imported into a staging area and the user is required to review it and accept it before it is saved into the study.

If the data looks correct, click Accept on the right of the observation tab. The data will be saved into the database.