Pedigree nurseries are managed through a series of nurseries facilitated through the Study Manager.

At the end of this tutorial, the user should be able to:

1. Create a nursery study for population development

2. Advance a pedigree nurseries by derivative methods

3. Withdraw seed from inventory and prepare planting labels

Open the Manage Studies application from the main menu and click on Start a new study. Name the nursery <your initials>21F1, CGM21F1 for me, and fill in the basic details of description and objective and select the study type Nursery.

You can add variables to the new study directly from the Ontology pick lists as we did for the crossing block in a previous Tutorial, or you can pick up all the variables from any previously used study. To use this option check the Use a previously created study as a template check box. Then choose the previous study which is similar to your current study:

The variables from the template study are imported into the new study. Change the settings if needed.

Save the study in your 2021 Nursery folder.
On the Germplasm and Checks tab, the extra variables for Cross and Seed source are also recovered from the template, so you just need to Browse for the list of planting material. The F1 list you made in the crossing Tutorial. U21CGMF1 in my case.
Mbe has been inherited from the template, and this location is still correct, but a new seeding date should be entered if known.
On the Experimental Design tab select Entry list order and Generate the design.

Once the nursery is planted and harvested we can advance the families to F2 seed by bulking seed from each F1 cross family. Select Actions>Advance study options>Advance study:

Choose the location to advance (we only have one), then select the advance method for all plots – Random Bulk - DRP. We will advance all plots so leave the All plots are selected checkbox ticked.

Click Finish.
Review the list of advanced lines, and if it looks correct, click Finish again.
Save the list with name <your initials>21F2 in your 2021 lists folder.

You can follow the steps in the tutorial Adding Inventory for a Harvest List to add inventory for the F2 seeds if you would like.

Follow the steps to create a new nursery for the F2 seeds. Use the F1 nursery as a template for planting at Mbe in Entry list order. (You can review the steps in the first section above).

In this nursery we will need to add a variable to record the number of plants selected from each F2 population.
If flowering date is not a column in the Observation sheet, click Add opposite Traits and search for flowering and add FlwDate_50Flw_Date as a trait.

Click the Add button opposite SELECTIONS on the Observation tab and look for the variable NPSEL to add to the fieldbook.

Now we can export the fieldbook for data collection. Select Actions>Data collection options>Export study book:

Choose Observations and then choose Excel Format.
You will download an excel fieldbook with a description sheet:

And an Observation Sheet. Fill in the data for the Flowering Date (dates are entered as numbers in format YYYMMDD) and the number of plants selected from each plot:

Back in BMS select Actions>Data collection options>Import observations. Press Continue for the observations sheet, then browse to the fieldbook file you just saved. When you have chosen the file click Import. The data will be imported into a staging area and the user is required to review it and accept it before it is saved into the study.

If the data looks correct, click Accept on the right of the observation tab. The data will be saved into the database.

Now select Actions>Advance study options>Advance study:

Click Continue to select the location, Mbe.






Choose the method Single Plant selection in the drop down box. Now this time we will not select the same number of lines from all plots, so uncheck that tickbox and then make sure that NPSEL is selected as the variable which defines the number of lines selected from each plot.

Click Finish.
Review the F3 lines and click Finish again then save the lines in an F3 list – CGM20F3 for me.

We follow the steps in tutorial Adding Inventory for a Harvest List to save inventory for the F3 harvest list.
Open the F2 nursery (CGM21F2 for me) and on the Crosses and Selections tab select all the entries.

From the Actions menu on the Crosses and Selections tab choose Create lots.
Fill in the Create Lot form with the Stock ID prefix (CGM for me), the storage location, and choose the units number of packets since we will not weigh the seed for each F3 line in this example. Since we know the number of packets, one for each line, and where they will be stored we can commit the inventory straight away by checking the Confirm transactions on saving check box. You can create labels for this inventory by following steps in the in tutorial Adding Inventory for a Harvest List.

If you go to the list manager (LISTS>Germplasm Lists) and open the F3 list you can see the inventory: