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  • Select Multi-Trait Query under Queries. Click Next.

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  • All phenotypic traits defined in the ontology are available for selection. Select the phenotypic traits to include in the query.

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To facilitate choosing among the available traits, they are organized into a tree starting with major groups such as 'Abiotic stress', 'Agronomic', 'Biotic stress', etc.

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  • Select Next. The system will search for all of the studies that contain observations for one or more of the traits chosen. Depending on the amount of the data in your database, the query may take several minutes to process.

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Three traits are selected; grain moisture (GmoiGMoi_NIRS_pct) , grain yield (GY_FW_kgPlot), and Asperillus flavus ear rot (AflavER_1_5). Grain moisture and yield are numeric variables and Asperillus flavus ear rot is a categorical variable.

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  • Include or exclude environments from query. Specify the importance of each location by weight: important, critical, desirable, or ignored.

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217 environments were evaluated for grain moisture (Gmoi_NIRS_pct) , grain yield (GY_FW_kgPlot), and Asperillus flavus ear rot (AflavER_1_5). In this example, all locations are included and all locations are considered important.

  • Filter trial environments by location by selecting Filter by Location and specifying the locations of interest. Apply.

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In this example, the locations have been limited to those in Eastern and Southern Africa.

 

  • Next.

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The original 217 environments have been reduced to 161 environments in Eastern and Southern Africa.

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  • Specify condition and weights for numeric traits.

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The numeric trait, grain moisture has been limited to those germplasm with greater than 20% moisture.

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  • Reduce the column widths to make the selection criteria visible.

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  • Specify condition and weights for categorical traits. Select Next. Use commas to separate multiple values. To enter a single value, just enter the value with no commas.

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Asperillus flavus ear rot (AflavER_1_5) has 5 categories. In this example, the results are restricted to those germplam with Asperillus flavus ear rot scores of highly resistant (1) and resistant (2).

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  • Click twice on the COMBINED SCORE column and the table to sort results in descending order from highest to lowest.

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Four germplasm have combined scores of 1.0 and will be selected for inclusion in an upcoming study.

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  • Tag germplasm of interest and Save to List.

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  • Specify the germplasm list details and Save. The germplasm list now available from Manage Germplasm and can be included as a study list.

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