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First we need to clarify the meaning of the following basic terms, which are used throughout this guide guideline and which are sometimes confused: treatments, factors, factor levels, and response variables
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For this exercise, the step by step of a 3 by 2 multifactorial study is decribeddescribed, where 3 germplasm entries (Variety Varieties A, b andCB and C) by 2 Nitrogen levels (0 and 150 kg/ha) are evaluted evaluated for grain yield response at 2 locations.
Open the Study Manager and click “Start a New Study”.
Enter mandatory information. Name, describe, and choose the study type. Set the file directory by selecting "Change Folder" option.
In the Germplasm and Check tab click “Browse” to select the germplasm list for use in the study.
In the Treatment Factors tab, click Add+ and select the treatment factor variable that defines the levels, NFert_NO for this example. Then display the Label dropdown menu and select the variable that labels each level treatment, NFert_kg_ha for this example. Finally complete Then specify the value label for each level, 0 and 150 for this example.
In the Environments tab, specified the number of environments for this study and then add Environmental details and Environmental conditions variables as needed for describing each location instance.
In the Experimental Design tab, import or generate the design, choose RCBD and generate it with 2 replications for this example.
After a successfully design generation, the study is ready to print labels, add traits to be collected, export electronic studybooks and import collected data. For this example, a grain yield response (GY_Calc_kgha) variable was added.
Importing an Externally Generated Multifactorial Study
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The following example shows the importation of how to import a study experimental design for 2 Locations, with a split plot design of two factors, 2 levels of Nitrogen fertilization (0 and 150 kg/ha) by 3 varieties (germplasm entries). In this case the Nitrogen fertilization is the factor also used as blocking BLOCK_NO (Main plot), and the germplasm entries correspond as the SUB_BLOCK factor.
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In the Experimental Design tab, click on “Or import an experimental design”, specify the file format and browse for the file template containing the experimental design.
Revise mapped and un-mapped variables, remap or map accordingly, then click Next.
Review that the Designation column was filled with the correct germplasm names and click Finish.
The Observation tab has now the imported experimental design and the study is ready to print for printing labels, add traits to be collected, export electronic studybooks and import collected data. The germplasm Designation column will be filled with the Preferred names of the entries added in the Germplasm & Checks tab.
Example template to import file for importing a split plot design as multifactorial 3 by 2, 3 entries by 2 N levels, in 2 Locations:
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